Chromatography and Transition Analysis play a pivotal role in various industries, particularly in the purification of substances and the separation of complex mixtures. However, ensuring the reliability and effectiveness of chromatography columns presents ongoing challenges. Over time, column substrates degrade, necessitating periodic refreshment to maintain optimal performance. Despite the significance of chromatography peaks in determining product quality and yield, comparing them accurately remains challenging. Additionally, monitoring the integrity and efficiency of chromatography columns requires specialized techniques to detect any deviations or anomalies that could impact results. Despite these obstacles, chromatography and transition analysis continue to be indispensable tools for achieving precise separations and purifications in diverse applications.